| [研究进展]何欣桐同学研究了益生菌表面覆盖率在与病原菌相互作用中的作用(相关成果发表在Langmuir) |
| 发布时间： 2024-02-26 浏览次数： 84 |
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Title: The Role of Surface Coverage of Sessile Probiotics in Their Interplay with Pathogen Bacteria Investigated by Digital Holographic Microscopy
Abstract: The adhesion of probiotics plays an important role in the gastrointestinal tract. Understanding the effect of the coverage of colonized probiotics on enteric pathogens is critical for designing effective probiotic therapies. In the present work, we have investigated the adaptive behaviors of an intestinal pathogenic bacteria Enterobacter sakazakii (ES) near the surfaces coated with a probiotic - Lactobacillus rhamnosus GG (LGG) as a function of surface coverage ratio (CRLGG) by using a home-setup digital holographic microscopy (DHM). It shows that ES cells can adaptively sense LGG within a distance of 4.2 μm even at CRLGG as lowas 0.05%. The growth inhibition of ES cells slightly varies with CRLGG, but the near-surface acceleration and accumulation of ES cells have much dependence on CRLGG.Namely, as CRLGG increases from 0.05% to 24.6%, the percentage of actively swimming ES, the motion bias, the acceleration and the interplay duration do not linearly vary with CRLGG. Instead, each of them shows an extreme at CRLGG of 13.4%, corresponding to the chemotaxis behaviors of ES cells induced by diffusing stimuli (organic acids, bacteriocins, etc.) released from LGG which showed an extreme of concentration gradient at CRLGG = 13.4% by simulations. Our study clearly demonstrates that surface coverage of sessile probiotics profoundly influences their interplay with pathogen bacteria, which should be taken into account in designing probiotics therapies. DOI：10.1021/acs.langmuir.3c02436 |
