姓名:罗剑飞 职称:副教授,硕士生导师 研究方向:环境微生物学、微生物生态学、环境微生物技术 电子邮箱:ljfjf2002@scut.edu.cn 办公地点:B6-301 |
微生物学
生物工程
2006.09-2011.07 华南理工大学, 发酵工程, 工学博士
2012.08 -至今 华南理工大学, 生物科学与工程土耳其里拉兑换人民币, 副教授
2011.10-2012.07 华南理工大学, 生物科学与工程土耳其里拉兑换人民币, 讲师
本科生教学:发酵工程、微生物生态学、生物工程设备、基础生命科学
研究生教学:微生物生理学
教学成果:华南理工大学青年教师本科课堂教学竞赛优胜奖(2020-2021学年度)、第十二届“挑战杯”广东省赛二等奖指导老师、大德赢新版app创新创业训练计划指导老师(2022国家级项目结题优秀)
研究兴趣
化能自养微生物——包括氨氧化菌(氨氧化细菌、氨氧化古菌、完全氨氧化菌)、亚硝酸盐氧化菌、硫氧化细菌等,在元素生物地球化学循环中扮演关键角色,在环境治理中发挥重要作用,但这些微生物目前多处于难/未培养状态,国内各微生物菌种保藏中心的缺乏相关保藏记录。我们基于研究与探索,获得这些难/未培养的微生物资源,为科学研究和自主知识产权技术的开发提供支持。
1.难/未培养化能自养菌的菌种:设计并优化富集策略和分离纯化方法,从环境中得到这些微生物,并在细胞和组学水平探讨它们新的代谢特性,挖掘隐藏于自然界中未知的微生物类群。
2.难/未培养化能自养菌的保存:探寻不同于常规微生物、适合化能自养菌的长期保存方法。
3.化能自养菌的生态效应:揭示化能自养菌与异养菌之间相互作用的基。/未培养化能自养菌的富集和分离、以及环境治理过程中化能自养菌功能的优化提供理论指导。
4.化能自养菌新功能的开发:基于菌株的新功能,结合主流污染物治理技术,开发或强化微生物介导的污染物治理方法。
承担的主要科研项目
1.国家自然科学基金面上项目:典型农田土壤中氨氧化古菌对氧化亚氮释放贡献度的新认识,2020-2023,主要参与人
2.广东省自然科学基金面上项目:氨氧化古菌氨氮耐受机制的研究,2021-2023,项目负责人
3.华南理工大学德赢新版app高校基本科研业务面上项目:尿素促进土壤氨氧化菌生长及其N2O排放的研究,2022-2023,项目负责人
4.国家自然科学基金重大研究计划培育项目:化能自养硫氧化细菌介导的硫氧化-固氮耦合反应驱动机制及生态效应研究,2020-2022,项目负责人
5.华南理工大学德赢新版app高校基本科研业务费重点项目:未培养微生物的“钓取”及研究—以氨氧化古菌为例,2019-2020,项目负责人
6.国家自然科学基金面上项目:氨氧化古菌对农田土壤氧化亚氮释放的贡献及其作用机制,2015-2018,主要参与人
7.国家自然科学基金青年基金项目:长期施化肥土壤中生物进化的研究,2014-2016,项目负责人
8.国家自然科学基金面上项目:寡营养淡水环境中氨氧化古菌及其氨氧化特性研究,2013-2016,主要参与人
代表性成果
代表性论文
32. Jiang Y#, Wu J#, Liu M, Zhu Y, Wen Q, Lin W*, Luo J*. Growth and N2O production of Nitrosocosmicus clade in agricultural soil when responding to high ammonium inputs. Journal of Soils and Sediments, 2023, https://doi.org/10.1007/s11368-023-03547-2 (JCR Q2, IF 3.536)
31. Mu D, Lin W*, Luo J*. on-negligible effect of native rhizobacteria on cooperation with plant growth regulators improve tolerance to cadmium: A case study using duckweed Spirodela polyrhiza as indicating plant. Journal of Plant Growth Regulation, 2023, https://doi.org/10.1007/s00344-023-10954-9 (JCR Q1, IF 4.64)
30. Liu B#, Lin W#, Huang S, Sun Q, Yin H, Luo J*. Removal of Mg2+ inhibition benefited the growth and isolation of ammonia-oxidizing bacteria: An inspiration from bacterial interaction. Science of the Total Environment, 2022, 838: 155923. (JCR Q1, IF 10.753)
29. Xie Z#, Wu F#, Lin W*, Luo J*. The utilization of photophosphorylation uncoupler to improve lipid production of Chlorella, a case study using transcriptome and functional gene expression analysis to reveal its mechanism. Biochemical Engineering Journal, 2022, 178: 108275. (JCR Q2, IF 4.446)
28. 曲珊珊, 严洪珊, 罗剑飞*, 林炜铁*. 化能自养硫氧化细菌Halothiobacillus sp. LS2介导的以乙炔为电子受体的硫氧化反应. 微生物学报, 2021, 61(6): 1488-1495.
27. Xie, Z, Lin, W*, Luo, J*. Co-cultivation of microalga and xylanolytic bacterium by a continuous two-step strategy to enhance algal lipid production. Bioresource Technology, 2021, 330: 124953. (JCR Q1, IF 9.642)
26. Liu, L, Liu, M, Jiang, Y, Lin, W*, Luo, J*. Production and excretion of polyamines to tolerate high ammonia, a case study on soil ammonia-oxidizing archaeon “Candidatus Nitrosocosmicus agrestis”. mSystems, 2021, 6: e01003-20. (JCR Q1, IF 6.496)
25. Xie, Z#, Li, S#, Lin, W*, Luo, J*. Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment. International Journal of Systematic and Evolutionary Microbiology, 2021, 71(2):004657. (JCR Q3, IF 2.747)
24. Dixit M, Liu H, Luo J, Shukla*. Effluents detoxification from pulp and paper industry using microbial engineering and advanced oxidation techniques. Journal of Hazardous Materials, 2020, 398: 122998. (JCR Q1, IF 9.038)
23. Xie, Z#, Lin W#, Liu J*, Luo J*. Mixotrophic cultivation of Chlorella for biomass production by using pH-stat culture medium: Glucose-Acetate-Phosphorus (GAP). Bioresource Technology, 2020, 313: 123506. (JCR Q1, IF 7.539)
22. Wang X#, Liu L#, Lin W*, Luo J*. Development and characterization of an aerobic bacterial consortium for autotrophic biodegradation of thiocyanate. Chemical Engineering Journal, 2020, 398: 125461. (JCR Q1, IF 10.652)
21. Hu J, Liu H, Shukal P, Lin W*, Luo J*. Nitrogen and phosphorus removals by the agar-immobilized Chlorella sacchrarophila with long-term preservation at room temperature. Chemosphere, 2020, 251: 126406. (JCR Q1, IF 5.778)
20. Mu D, Liu H, Lin W*, Shukla P*, Luo J*. Simultaneous biohydrogen production from dark fermentation of duckweed and waste utilization for microalgal lipid production. Bioresource Technology, 2020, 302: 122879. (JCR Q1, IF 7.539)
19. Liu L, Li S, Han J, Lin W*, Luo J*. A two-step strategy for the rapid enrichment of Nitrosocosmicus-like ammonia-oxidizing thaumarchaea. Frontiers in Microbiology, 2019, 10: 875. (JCR Q1, IF 4.259)
18. Liu K, Han J, Li S, Lin W*, Luo J*. Insight into the diversity of antibiotic resistance genes in the intestinal bacteria of shrimp Penaeus vannamei by culture-dependent and independent approaches. Ecotoxicology and Environmental Safety, 2019, 172: 451-459. (JCR Q1, IF 4.527)
17. Ban S, Lin W*, Luo Z, Luo J*. Improving hydrogen production of Chlamydomonas reinhardtii by reducing chlorophyll content via atmospheric and room temperature plasma. Bioresource Technology, 2019, 275: 425-429.
16. Ban S, Lin W*, Luo J*. Ca2+ enhances algal photolysis hydrogen production by improving the direct and indirect pathways. International Journal of Hydrogen Energy, 2019, 44: 1466-1473.
15. Tu Z#, Liu L#, Lin W#, Xie Z, Luo J*. Potential of using sodium bicarbonate as external carbon source to cultivate microalga in non-sterile condition. Bioresource Technology, 2018, 266: 109-115.
14. Lin W, Zhao D, Luo J*. Distribution of alkaline phosphatase genes in cyanobacteria and the role of alkaline phosphatase on the acquisition of phosphorus from dissolved organic phosphorus for cyanobacterial growth. Journal of Applied Phycology, 2018, 30: 839-850.
13. Ban S#, Lin W#, Wu F, Luo J*. Algal-bacterial cooperation improves algal photolysis-mediated hydrogen production. Bioresource Technology, 2018, 251: 350-357.
12. Luo J, Tan X, Liu K, Lin W*. Survey of sulfur-oxidizing bacterial community in the Pearl River water using soxB, sqr, and dsrA as molecular biomarkers.3 BIOTECH, 2018, 8:73.
11. Xie Z#, Lin W#, Luo J*. Comparative phenotype and genome analysis of Cellvibrio sp. PR1, a xylanolytic and agarolytic bacterium from the Pearl River. BioMed Research International, 2017, 6304248.
10. Lin W, Li P, Liao Z, Luo J*. Detoxification of ammonium to Nannochloropsis oculata and enhancement of lipid production by mixotrophic growth with acetate. Bioresource Technology, 2017, 227: 404-407.
9. Xie Z#, Lin W#, Luo J*. Promotion of microalgal growth by co-culturing with Cellvibrio pealriver using xylan as feedstock. Bioresource Technology, 2016, 200: 1050-1054.
8. Xie Z, Lin W*, Luo J*. Genome sequence of Cellvibrio pealriver PR1, a xylanolytic and agarolytic bacterium isolated from freshwater. Journal of Biotechnology, 2015, 214: 57-58.
7. Chen L#, Luo J#, Li P, Yu H, Huang J, Luo L*. Microbial diversity and flavor formation in onion fermentation. Food & function, 2014, 5: 2338-2347.
6. Luo J, Wang Y, Tang S, Liang J, Lin W*, Luo L*. Isolation and Identification of Algicidal Compound from Streptomyces and Algicidal Mechanism to Microcystis aeruginosa. PLoS ONE 2013, 8: e76444.
5. Luo J, Tian G, Lin W*. Enrichment, isolation and identification of sulfur-oxidizing bacteria from sulfide removing bioreactor. Journal of Environmental Sciences, 2013, 25: 1393-1399.
4. Luo J, Lin W*, Cai X, Li J. Optimization of fermentation media for enhancing nitrite-oxiding activity by artificial neural network coupling genetic algorithm. Chinese Journal of Chemical Engineering, 2012, 20: 950-957.
3. Luo J, Lin W*, Guo Y. Functional genes based analysis of sulfur-oxidizing bacteria community in sulfide removing bioreactor. Applied Microbiology and Biotechnology, 2011, 90: 769-778.
2. Lin W, Luo J, Guo Y. Comparison and characterization of microbial communities in sulfide-rich wastewater with and without Propidium Monoazide treatment. Current Microbiology, 2011, 62: 374-381.
1. Luo J, Lin W*, Guo Y. Method to detect only viable cells in microbiology. Applied Microbiology and Biotechnology, 2010, 86: 377-384.
授权专利
13. 罗剑飞, 林炜铁, 刘步蟾. 一种培养基及其在分离纯化培养氨氧化细菌中的应用, 202111071843.7
12. 罗剑飞, 林炜铁, 胡骏. 一种利用琼脂包埋保存的微藻处理氮磷废水的方法, 201911074056.0
11. 罗剑飞, 林炜铁, 谢章彰, 陈伟峰. 一种通过稳定pH快速培养小球藻的培养基及其应用, 201910098697.3 (授权)
10. 林炜铁, 罗剑飞, 刘亮霆. 一种从环境中快速富集Nitrosocosmicus属氨氧化古菌的方法, 201811591712.X
9. 林炜铁, 罗剑飞, 黄永泉, 党通州. 一种利用电导率检测植物细胞破壁率的方法, 201710377004.5
8. 罗剑飞, 林炜铁, 谢章彰. 一种共培养促进微藻快速生长的方法, 201510872999.3
7. 林炜铁, 吴晓壬, 罗剑飞. 一种有机溶剂提高发光细菌检测毒性物质敏感度的方法. 201610968482.9
6. 林炜铁, 罗剑飞, 谢章彰. 一种产琼胶酶、淀粉酶和木聚糖酶的纤维弧菌, 201510872308.X
5. 罗剑飞, 林炜铁, 谢章彰. 一种利用秸秆半纤维素培养小球藻的方法, 201510858635.X
4. 林炜铁, 罗剑飞, 杨晓苹. 一种检测普洱茶微生物群落结构的方法, 201310020829.3
3. 林炜铁, 李会琴, 昝继清, 罗剑飞. 一种微生物絮凝剂的生产方法, 200910042265.7
2. 林炜铁, 梁锏文, 罗剑飞, 袁慧明. 一种去除淡水中水华蓝藻的方法, 200910037692.6
1. 林炜铁, 崔华平, 朱雅楠, 罗剑飞.一种固定硝化细菌降解养殖废水亚硝酸盐的工艺, 200810198302.9 (授权)